Beneficial Role of Platelet –Rich Plasma (PRP) combined with Glass Wool Filtration for Best Recovery of Spermatozoa in Infertile Men


  •   Watfaa A. Abduljabar

  •   Hayder A. L. Mossa

  •   Muayad S. Abood


Background: Platelet –Rich Plasma (PRP) is a novel therapeutic agent used in multiple medical fields and one of these fields is the reproduction for best spermatozoa preparation and activation for upgrading activity and motility of the spermatozoa and filtered with glass wool filtration to eliminate any round cells and leukocytes from the sample.

Objectives: The aim of this research is to study some sperm characteristics in two groups, the normozoospermic infertile men and th asthenozoospermic infertile group before and after activation with Glass wool Filtration and PRP and compare between both.

Patients and Methods: In this study 60 infertile men were enrolled and divided into 2 groups,15 normozoospermic infertile men, and 45 ashenozoospermic infertile men during their attendance to the Infertility Clinic in High Institute for Infertility Diagnosis and Assisted Reproductive Technologies Al-Nahrain University. The collected semen samples, and seminal fluid analysis were assessed, each semen sample was divided into 3 tubes and labelled as the 1st tube which was before activation, the 2nd tube was after glass wool filtration activation technique and the 3rd tube was for semen activated by adding PRP to the medium used for glass wool filtration activation.

Results: Both techniques showed increased and improved sperm motility, but the PRP was superior to the glass wool alone in upgrading Sperm Grade A Motility %, Sperm Grade B Motility % and decreasing Sperm Grade C Motility %, and Sperm Grade D Motility (Immotile Sperm).

Conclusion: The PRP was significantly effective in improving the sperm activity and upgrading sperm motility more than Glass Wool Filtration technique.

Keywords: Glass Wool Filtration (GWF), Platelet –Rich Plasma (PRP)


S. S. Al-Dujaily, H. A. L. Mossa and S. N. Alwachi, “Study of Calpain Activity, Sperm Penetration Assay in Unexplained Infertile Men in Relevance to Assisted Reproductive Technologies,” World Journal of Pharmaceutical Research, 4(2):100-117, 2015.

E. E Puscheck and R. S. Lucidi, “Infertility Practice, Essential Overview,” Etiology of Infertility, 25, 2020.

S. Barak and H. W. Gordon Baker, “Clinical management of male infertility,” Endocrinology: Adult and Pediatric. 7th ed. Philadelphia, PA: Elsevier Saunders; chap 141, 2016.

A. S. Hindal, H. A. L. Mossa and M. S. Abood, “Reactive Oxygen Species Levels in Seminal Plasma in a Sample of Iraqi

Infertile Men using Advanced Stimulatory Method for Activation of Spermatozoa,” International Journal of Medical Research & Health Sciences, 7(12): 51 – 55, 2018.

E. Ali, U. AL-Kawaz and M. B. Fakhrildin, “A New sperm Preparation Technique by Combination of Density Gradient Centrifugation and Glass Wool Filtration,” International Journal of Advanced Research, 4(4):1112-1115, 2004

World Health Organization, “Laboratory Examination and Processing of Human Semen,” 5th edition, 2010.

M. Punab, O. Poolamets and P. Paju, “Causes of male infertility: a 9-year prospective monocentre study on 1737 patients with reduced total sperm counts,” Hum Reprod 32: 18-31, 2017.

Y. Terada, G. Schatten, H. Hasegawa, and N. Yaegashi, “Essential roles of the sperm centrosome in human fertilization: developing the therapy for fertilization failure due to sperm centrosomal dysfunction,” The Tohoku Journal of Experimental Medicine, 220(4), 247-258, 2010.

C. J. De Jonge, “Sperm preparation for IVF and ICSI,” In Handbook of IVF ,4th edition, 145-156, 2017.

A. A. Kadhim, H. A. Mossa and M. O. A. Selman, “New Sperm Preparation Technique by Glass Wool Filtration Combined with Pentoxifylline Techniques versus Glass Wool Filtration alone for Infertile and Fertile Men,” Iraqi Journal of Embryos and Infertility Researches 7(1): 28-36, 2017.

Z. P. Nagy, A. C. Varghese and A. Agarwal, “Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices,” Springer Science& Business Media, 2012.

A. A. Kadhim, H. A. Mossa and M. S. Abbood, “A comparison of new sperm preparation technique by glass wool filtration combined with pentoxifylline with other techniques in asthenozoospermic men,” International Journal of Advanced Research, 5(4): 1178–1182, 2017.

N. A. Hamza, M. O. Selman and H. A. Mossa, “Comparison of Best Yield of in vitro Sperm activation Techniques with New technique of Caffeine Combined with Density Gradient Centrifugation in Iraqi Patients,” Journal of Pharmaceutical Sciences and Research, 10(1): 36-39, 2018.

S. G. Boswell, B. J. Cole, E. A. Sundman, V. Karas and L. A. Fortier, “Platelet-rich plasma: a milieu of bioactive factors,” Arthroscopy, 28:429–439, 2012.

L. F. Marques, T. Stessuk, I. C. Camargo, N. Sabeh Junior, L. dos Santos and J. T. Ribeiro-Paes, “Platelet-rich plasma (PRP): methodological aspects and clinical applications,” Platelets, 26:101–113, 2015.

M. D. Wild and W. E. Roudebush, “Platelet-activating factor improves intrauterine insemination outcome,” Am J Obstet Gynecol, 184:1064-1065, 2001.

B. S. Minhans, R. Kumar, D. D. Ricker, J. L. Robertson and M. G. Dodson, “The presence of platelet activating factor- like activity in human spermatozoa,” Fertil Steril, 55(2):372-6, 1991.


How to Cite
Abduljabar, W. A., Mossa, H. A. L., & Abood, M. S. (2021). Beneficial Role of Platelet –Rich Plasma (PRP) combined with Glass Wool Filtration for Best Recovery of Spermatozoa in Infertile Men. European Journal of Clinical Medicine, 2(5), 11-13.