Immunoreactivity against Dermatophagoides pteronyssinus Assessed by the Leukocyte Adherence Inhibition Test in Patients with Intrinsic Atopic Dermatitis and Correlated “Intrinsic” Non–IgE-mediated Allergic Conditions


  •   Celso Eduardo Olivier

  •   Daiana G. Pinto

  •   Ana P. M. Teixeira

  •   Jhéssica L. S. Santana

  •   Raquel A. P. G. Santos

  •   Regiane P. S. Lima


Background: Due to the lack of standardized laboratory procedures able to demonstrate specific immune responses against the culprit allergens, the non—IgE-mediated allergy syndromes are a group of conditions diagnosed mostly by clinical examination and exclusion criteria.

Objective: To evaluate the opportunity of the Leukocyte Adherence Inhibition Test (LAIT) to discriminate specific immunoreactivity against Dermatophagoides pteronyssinus (Dp) in a group of patients with non–IgE-mediated chronic allergic conditions.

Methods: Ex vivo challenge tests performed with Dp were monitored by LAIT in patients presenting diverse non–IgE-mediated allergic conditions: intrinsic Atopic Dermatitis (iAD), intrinsic Allergic Rhinitis (iAR), intrinsic Ocular Allergy (iOA), intrinsic Chronic Pharyngitis (iCP), and intrinsic Asthma (iAS).

Results: The mean LAI of the control group was 7%; the mean LAI of the iAR group was 34%; the mean LAI of the iCP group was 44%; the mean LAI of the iAS group was 45%; the mean LAI of the iOA group was 47%; the mean LAI of the iAD group was 55%. The non-parametric Wilcoxon-Mann-Whitney U test comparing the control group with each other group showed significance with p-value < α = 0.05 for all groups.

Conclusion: The Leukocyte Adherence Inhibition Test is an easy, quick, and inexpensive ex vivo immunoassay with the potential to predict individual immunoreactivity against HDM allergens in real-world patients with non–IgE-mediated allergies.

Keywords: Allergens, allergic conjunctivitis, allergic rhinitis, antigen, asthma, atopic dermatitis, Dermatophagoides pteronyssinus, leukocyte adherence inhibition test, immunoassay, hypersensitivity, pharyngitis


Gell PGH, Coombs RRA. Classification of allergic reactions responsible for clinical hypersensitivity and disease. In: Gell PGH, Coombs RRA (eds) Clinical Aspects of Immunology. Blackwell Scientific Publications, Oxford, 1968: 575-596.

Olivier CE, Pinto DG, Lima RPdS, Teixeira APM, Santana JLS. Self-imposed food restriction and oral food challenges are correlated with precipitin's accuracy in the diagnosis of non–IgE-mediated food-related adulthood acute episodes of urticaria. Journal of Allergy & Therapy. 2021; 12(8): 1-8.

Olivier CE Food Allergy. Journal of Allergy & Therapy. 2013; S3: 004: 1-7.

Finkelman FD, Khodoun MV, Strait R. Human IgE-independent systemic anaphylaxis. J Allergy Clin Immunol. 2016; 137(6): 1674-1680.

Vasudev M, Cheung DS, Pincsak H, Li SH, Yan K, Simpson P, et al. Expression of high-affinity IgE receptor on human peripheral blood dendritic cells in children. PLoS ONE.2012; 7(2): 23.

Sihra BS, Kon OM, Grant JA, Kay AB. Expression of high-affinity IgE receptors (Fc epsilon RI) on peripheral blood basophils, monocytes, and eosinophils in atopic and nonatopic subjects: relationship to total serum IgE concentrations. J Allergy Clin Immunol. 1997; 99(5): 699-706.

Mora J, Riggs EK, Fu J, MacGlashan DW, Jr., Fox SA, Yu B, et al. Expression of the high affinity IgE receptor by neutrophils of individuals with allergic asthma is both minimal and insensitive to regulation by serum IgE. Clinical immunology. 2019; 132(1): 132-140.

Joseph M, Gounni AS, Kusnierz JP, Vorng H, Sarfati M, Kinet JP, et al. Expression and functions of the high-affinity IgE receptor on human platelets and megakaryocyte precursors. Eur J Immunol. 1997; 27(9): 2212-2218.

Engeroff P, Vogel M. The role of CD23 in the regulation of allergic responses. Allergy. 2007; 76(7): 1981-1989.

Qiu C, Zhong L, Huang C, Long J, Ye X, Wu J, et al. Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients. Scientific Reports. 2010; 10(1): 4700.

Brostoff J, Johns Dennis P, Stanworth R. Complexed IgE in atopy. The Lancet. 1997; 310(8041): 741-742.

Brostoff J, Carini C, Wraith DG, Johns P. Production of IgE complexes by allergen challenge in atopic patients and the effect of sodium cromoglycate. Lancet. 1979; 1(8129): 1268-1270.

Tantilipikorn P, Siriboonkoom P, Sookrung N, Thianboonsong A, Suwanwech T, Pinkaew B, et al. Prevalence of local allergic rhinitis to Dermatophagoides pteronyssinus in chronic rhinitis with negative skin prick test. Asian Pac J Allergy Immunol. 2021; 39(2): 111-116.

Kuljanac I. The role of Dermatophagoides pteronyssinus in atopic dermatitis. Acta Dermatovenerol Croat. 2006; 14(2): 86-89.

Liao EC, Ho CM, Lin MY, Tsai JJ. Dermatophagoides pteronyssinus and Tyrophagus putrescentiae allergy in allergic rhinitis caused by cross-reactivity not dual-sensitization. J Clin Immunol. 2010; 30(6): 830-839.

Leonardi A, Silva D, Perez Formigo D, Bozkurt B, Sharma V, Allegri P, et al. Management of ocular allergy. Allergy. 2006; 74(9): 1611-1630.

Vidal C, Lojo S, Juangorena M, Gonzalez-Quintela A. Association Between Asthma and Sensitization to Allergens of Dermatophagoides pteronyssinus. J Investig Allergol Clin Immunol. 2016; 26(5): 304-309.

Kerschenlohr K, Decard S, Darsow U, Ollert M, Wollenberg A. Clinical and immunologic reactivity to aeroallergens in "intrinsic" atopic dermatitis patients. J Allergy Clin Immunol. 2003; 111(1): 195-7.

Ingordo V, D'Andria G, D'Andria C, Tortora A. Results of atopy patch tests with house dust mites in adults with 'intrinsic' and 'extrinsic' atopic dermatitis. J Eur Acad Dermatol Venereol. 2002; 16(5): 450-454.

Halliday WJ. Historical Background and Aspects of the Mechanism of Leukocyte Adherence Inhibition. Cancer Res. 1979; 39(2): 558-563.

Kuratsuji T. Studies on leukocyte adherence inhibition test. Part II. Clinical applications of LAI test to detect delayed type hypersensitivity in infants and children. Keio J Med. 1981; 30(2): 65-69.

Hewitt CRA, Horton H, Jones RM, Pritchard DI. Heterogeneous proteolytic specificity and activity of the house dust mite proteinase allergen Der p I. Clinical & Experimental Allergy. 1997; 27(2): 201-207.

Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, et al. Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. The Journal of clinical investigation 104. 1999; (1): 123-133.

Keglowich L, Tamm M, Zhong J, Miglino N, Borger P. Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78/CXCL5 and Reduces Neutrophil Migration. Journal of Allergy. 2014: 673673.

Powell AE, Birch RE, Murrell H, Sloss AM. Cell populations in leucocyte adherence inhibition: requirement for T lymphocytes with IgG Fc receptors. Immunology. 1982; 46(4):689-696.

Kotlar HK, Sanner T. Role of circulating antibodies in the humoral leukocyte adherence inhibition response of lung and breast cancer patients. Cancer Lett. 1980; 11(1): 11-19.

Kotlar HK, Sanner T. Role of immune complexes in the humoral leukocyte adherence inhibition test. Cancer Immunology, Immunotherapy. 1981; 11(2): 109-113.

Olivier CE, Lima RPdS, Pinto DG, Santos RAPGd. The plasma preincubation with papain before the assay suggests that a gell and coombs type ii reaction is been demonstrated by the leukocyte adherence inhibition test. biomedical. Journal of Scientific & Technical Research. 2021; 36(3): 28647 - 28655.

World Medical Association Declaration of Helsinki. Ethical principles for medical research involving human subjects. JAMA. 2013; 310(20): 2191-2194.

Olivier CE, Argentão DGP, Santos RAPG, Silva MD, Lima RPS, Zollner RL. Skin scrape test: an inexpensive and painless skin test for recognition of immediate hypersensitivity in children and adults. Open Allergy Journal 2013; 6: 9-17.

Stephenson KN. Acute and chronic pharyngitis across the lifespan. Lippincotts Prim Care Pract. 2000; 4(5): 471-489.

Li Z, Huang J, Hu Z. Screening and diagnosis of chronic pharyngitis based on deep learning. Int J Environ Res Public Health. 2019; 16(10).

Hollender AR. Hypertrophy of the lingual tonsil and lymphoid tissue of the pharynx; reduction by electro-coagulation. Laryngoscope. 1932; 42(8): 622-626.

Olivier CE, Pinto DG, Lima RPS, Silva MDd, Santos RAPG, Teixeira APM, Simioni PU. Assessment of immunoreactivity against therapeutic options employing the leukocyte adherence inhibition test as a tool for precision medicine. European Journal of Clinical Medicine. 2021; 2(3): 40-45.

Kim H-Y. Statistical notes for clinical researchers: Nonparametric statistical methods: 1. Nonparametric methods for comparing two groups. Restorative dentistry & endodontics. 2014; 39(3): 235-239.

Fay MP, Proschan MA. Wilcoxon-Mann-Whitney or t-test? On assumptions for hypothesis tests and multiple interpretations of decision rules. Stat Surv. 2010; 4: 1-39.

Dunn IS, Halliday WJ. Interactions between T and B lymphocytes and macrophages in the production of leukocyte adherence inhibition factor. Cellular Immunology. 1980; 52(1): 48-61.

Haw WY, Polak ME, McGuire C, Erlewyn-Lajeunesse M, Ardern-Jones MR. In vitro rapid diagnostic tests for severe drug hypersensitivity reactions in children. Ann Allergy Asthma Immunol. 2016; 117(1): 61-66.

Fink A, Shahin R, Eliraz A, Bibi H, Berkenstadt H, Levin S, et al. Interferon modulates the leukotriene C4-induced non-adherence properties of leukocytes: acquisition of an asthmatic phenotype. Immunol Lett. 1985; 10(3-4): 159-163.

Fink A, Bibi H, Eliraz A, Tabachnik E, Bentwich Z. Leukotrienes (LTC4, LTD4) confer glass non-adherence on leukocytes of asthmatic individuals. Dependency on cyclooxygenase products and calcium ion. Immunol Lett. 1985; 10(6): 319-323.


How to Cite
Olivier, C. E., Pinto, D. G., Teixeira, A. P. M., Santana, J. L. S., Santos, R. A. P. G., & Lima, R. P. S. (2021). Immunoreactivity against Dermatophagoides pteronyssinus Assessed by the Leukocyte Adherence Inhibition Test in Patients with Intrinsic Atopic Dermatitis and Correlated “Intrinsic” Non–IgE-mediated Allergic Conditions. European Journal of Clinical Medicine, 2(6), 45–50.

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