Determination of Dynamic Plasma Thiol -disulfide Homeostasis with a Novel Technique in Intestinal Ischemia Reperfusion Injury


  •   Ersin Gürkan Dumlu

  •   Mehmet Tokaç

  •   Mustafa Özsoy

  •   İbrahim Kılınç

  •   Nazmiye Dinçer

  •   Özcan Erel

  •   Mehmet Kılıç


Introduction: Intestinal ischemia/reperfusion (I/R) is a serious life-threatening clinical case. Overproduction of reactive oxygen species (ROS) associated with oxidative stress plays a key role in I/R pathophysiology.

Objective: The aim of this study is to evaluate dynamic thiol / disulfide hemostasis in intestinal I/R in rats.

Materials and Methods: 24 Winstar albino rats were divided into 3 groups (8 animals in each group: (1) sham (operation without ischemia), (2) ischemia (90 minutes ischemia), (3) ischemia/reperfusion (after ischemia for 30 minutes reperfusion for 60 minutes). At the end of the process, liver samples were evaluated pathologically. Also, in plasma samples, native thiol, disulphide and total thiol levels were measured.

Results: Native thiol levels in ischemia and I/R group were found to be significantly lower compared to the sham group (P<0.001). Disulfide levels in Ischemia group were found to be significantly higher compared to the sham group (P< 0.05). Disulfide / native thiol levels in ischemia and I/R group were found to be significantly higher compared to the sham group (P < 0.05). Disulfide / total thiol levels in both ischemia and I/R group were found to be significantly higher compared to the sham group (P< 0.05).

Conclusion: Balance of dynamic thiol/disulfide is a quite suitable indicator for evaluating oxidative stress which occurs as a result of intestinal I/R. As a result, measurement of dynamic thiol/disulfide balance with Erel method is fully automated and practical for evaluating oxidative environment which is formed with ischemia reperfusion.

Keywords: thiol, disulfide, thiol / disulfide hemostasis, intestinal ischemia


Mallick IH, Yang W, Winslet MC, Seifalian AM. İskemi-reperfusion injury of the intestine and protective strategies against injury. Dig Dis Sci. 2004; 49: 1359-77.

Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol. 2000; 190: 255-66.

Parks DA, Granger DN. Contributions of iskemi and reperfusion to mucosal lesion formation. Am J Physiol 1986; 250: G749-G753.

Cerqueira NF, Hussni CA, Yoshida WB. Pathophysiology of mesenteric iskemi/reperfusion: a review. Acta Cir Bras 2005; 20: 336-343.

Nunomura A, Castellani RJ, Zhu X, Moreira PI, Perry G, Smith MA. Involvement of oxidative stress in Alzheimer disease. J Neuropathol Exp Neurol 2006; 65: 631–641.

Tunc T, Oter S, Güven A, Topal T, Kul M, Korkmaz A, Ongürü O, Sarici U. Protective effect of sulfhydryl-containing antioxidants against iskemi/reperfusion injury of prepubertal rat intestine. J Gastroenterol Hepatol. 2009; 24: 681-7.

Tokaç M, Taner G, Aydın S, Ozkardeş AB, Dündar HZ, Taşlıpınar MY, Arıkök AT, Kılıç M, Başaran AA, Basaran N Protective effects of curcumin against oxidative stress parameters and DNA damage in the livers and kidneys of rats with biliary obstruction. Food Chem Toxicol. 2013; 61: 28-35.

Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol. 1990; 186: 464–78.

Adams JD Jr, Lauterburg BJ, Mitchell JR. Plasma glutathione and glutathione disulfide in the rat: Regulation and response to oxidative stress. J Pharmacol Exp Ther 1983; 227:749.

Briviba K, Sies H. Non enzymatic antioxidant defense system. In: Frei B, ed. Natural antioxidants in human health and dis- ease. San Diego: Academic Press, 1994:107–128.

Sen CK, Packer L.Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr. 2000; 72: 653S-69S.

Erel O, Neselioglu S A novel and automated assay for thiol/disulphide e homeostasis. Clin Biochem. 2014; 47: 326-32.

Circu ML, Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med. 2010; 48: 749-62.

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th ed. Washington (DC): National Academies Press (US); 2011.

Slone EA, Rope MR, Roth M, Welti R, Fleming SD. TLR9 is dispensable for intestinal iskemi/reperfusion–induced tis- sue damage. Am J Clin Exp Immunol 2012; 1: 124–135.

Dumlu EG, Bozkurt B, Tokaç M, Kiyak G, Ozkardeş AB, Ergin M, Yazgan A, Kılıç M. Oxidative Stress and Lipid Peroxidation in the Ischemic Small Intestine: Pathological and Biochemical Evaluation in a Rat model of Superior Mesenteric Ischemia. Int Surg. 2014; 99: 868-74.

Boybeyi Ö, Gunal YD, Atasoy P, Kısa U, Aslan MK The effect of colchicine and low-dose methotrexate on intestinal iskemi/reperfusion injury in an experimental model. J Pediatr Surg. 2014; 49: 1471-4.

Zabot GP, Carvalhal GF, Marroni NP, Hartmann RM, da Silva VD, Fillmann HS. Glutamine prevents oxidative stress in a model of mesenteric iskemi and reperfusion. World J Gastroenterol. 2014; 20: 11406-14.

Karakaş BR, Davran F, Elpek GÖ, Akbaş SH, Gülkesen KH, Bülbüller N. The effects of luteolin on the intestinal iskemi/reperfusion injury in mice.J Invest Surg. 2014; 27: 249- 255.

Sizlan A, Guven A, Uysal B, Yanarates O, Atim A, Oztas E, Cosar A, Korkmaz A. Proanthocyanidin protects intestine and remote organs against mesenteric iskemi/reperfusion injury.World J Surg. 2009; 33: 1384-91.

Yucel AF, Kanter M, Pergel A, Erboga M, Guzel A. The role of curcumin on intestinal oxidative stress, cell proliferation and apoptosis after iskemi/reperfusion injury in rats. J Mol Histol. 2011; 42: 579-87.

Ypsilantis P, Lambropoulou M, Anagnostopoulos C, Tentes I, Tsigalou C, Pitiakoudis M, Kortsaris A, Papadopoulos N, Simopoulos C. Mesna preserves hepatocyte regenerating capacity following liver radiofrequency ablation under Pringle maneuver.J Surg Res. 2011; 169: 44-50.

Suyavaran A, Ramamurthy C, Mareeswaran R, Subastri A, Lokeswara Rao P, Thirunavukkarasu C. TNF-α suppression by glutathione preconditioning attenuates hepatic iskemi reperfusion injury in young and aged rats. Inflamm Res. 2015; 64(1): 71-81.

Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med. 2013; 65: 244-253.

Głowacki R, Bald E. Fully automated method for simultaneous determination of total cysteine, cysteinylglycine, glutathione and homocysteine in plasma by HPLC with UV absorbance detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2009; 877: 3400-3404.

Carru C, Deiana L, Sotgia S, Pes GM, Zinellu A Plasma thiols redox status by laser-induced fluorescence capillary electrophoresis.. Electrophoresis. 2004; 25: 882-889.


How to Cite
Dumlu, E. G., Tokaç, M., Özsoy, M., Kılınç, İbrahim, Dinçer, N., Erel, Özcan, & Kılıç, M. (2021). Determination of Dynamic Plasma Thiol -disulfide Homeostasis with a Novel Technique in Intestinal Ischemia Reperfusion Injury. European Journal of Clinical Medicine, 2(3), 55–59.